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. 2014 Jul 19;20(1):73–84. doi: 10.1007/s12192-014-0527-2

Table 1.

List of primers used. The qPCR primers were named according to the gene name

Sl. no. Gene Sequence (5′→3′) Ta (°C) Product length (bp) Reference/source
1 HSPA90 Forward- ACCAAAGCCGACCTCATC 54 121 Das et al. in manuscript
Reverse- AGAAACCCACGCCAAACT
2 HSPA70 Forward- CTACTCGGACAATCAGCC 54 105 Das et al. in manuscript
Reverse- GGAATGCCAATCAACTCA
3 HSPA30 Forward- TGACGCTGGACACTAAAGAC 58 163 Das et al. in manuscript
Reverse- CTTGAGGCAGATCAAACACTC
4 HSPA9 Forward- GCTGGTCGGAGGAATGA 53 87 Contig 87912
Reverse- GGGTTCACGGACTTACTGG
5 HSPA8 Forward- TCCCAAGGTCCAGGTT 51 114 Contig 110387
Reverse- GTCTTTCCCAGGTAGGC
6 HSPA5 Forward- GCGGTTTCCCTGGTCATT 53 172 Contig 54182
Reverse- GCGATTGCTTTGCCTGTT
7 HSPA4 Forward- CCCATTCAAGAGCGATAC 51 167 Contig 54154
Reverse- TTCACCATTCTGTCCACC
8 β-actin Forward- TTGGCAATGAGAGGTTCAGGT All above 153 Robinson et al. 2012
Reverse- TTGGCATACAGGTCCTTACGG
9 GRP78 Forward- ATGCGATTGCTTTGCCTGTT 60 1,010 Forward: Contig 54182
Reverse: Contig 52647
Reverse-ATGGTGGAGCGGAACAAG