Fig. 6.

SAHA-induced cleavage of HSP90 needs newly synthesized proteins. a–b K562 cells were untreated or treated with the indicated doses of cycloheximide (CHX) for 1 h, followed by treatment with SAHA (5 μM). a The cells were incubated with 10 μM fluorescent probe DCF-DA for 20 min and visualized using fluorescence microscopy. Scale bar: 40 μM. b The cell lysates were subjected to Western blot analysis