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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Science. 2013 Jul 19;341(6143):278–281. doi: 10.1126/science.1232995

Fig. 2. Regulation of MC4R byMRAP2a.

Fig. 2

(A) Surface expression of MC4R measured by whole-cell enzyme-linked immunosorbent assay (ELISA) in nonpermeabilized HEK293T cells transfected with mc4r and increasing amounts of mrap2a. (B) Europium-labeled NDP–α-MSH binding in HEK293T cells transfected with mc4r and increasing amounts of mrap2a. (C) Competition binding assay in HEK293T cells transfected with mc4r without or with mrap2a at mc4r/mrap2a ratios of 1:1 or 1:6. (D) Concentration-response curves of α-MSH–induced cAMP production in HEK293T cells expressing the CRE-luciferase reporter, mc4r, and different amounts of mrap2a. ***P < 0.001.