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. 2013 Nov 6;29(5):1295–1309. doi: 10.1002/jbmr.2141

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© 2014 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 7 — CD99 regulates RUNX2 activity modulating ERK 1/2 signaling. (A) Expression of CD99 and pERK 1/2 was analyzed by Westen blotting after CD99 silencing for 48 hours by siRNA (40 nM). Cells were transfected by a scrambled (SCR) and a siRNA against p53 (siRNA CTR) as controls at the same concentration. (B, C) Expression of active ERK 1/2 and RUNX2 was evaluated by immunofluorescence in CD99-expressing cells in the same conditions described before. Scale bar = 20 µm. (D) After CD99 silencing in OS cells for 48 hours, the expression of osteoblast differentiation markers was analyzed: collagen 1 and ALP decreased, as shown by qRT-PCR and by histochemical analysis.