Figure 3. Effect of Temperature on Antimicrobial Activity.

Aliquots of the culture supernatant were prepared in screw capped tubes, with pH adjusted to 7, and placed in water bath at 4, 35, 40, 50, 60, 70, 80 and 100°C for two hours. The antimicrobial compound was also subjected to 121°C for 15minutes. Before and after the treatment, the samples were tested for antimicrobial activity against B. subtilis as the indicator organism; an untreated cell-free supernatant served as the control negative.