Fig. 7.

In vivo and ex vivo fluorescence and polarization endoscopy of wound healing inflammation model. (a) Ex vivo brightfield images of wounds created via endoscopy procedure in vivo. (i,ii) Wound bed at day 4 postendoscopic injury. Dotted red line marks the neutrophil cap formed as a result of inflammation. (iii) RGB color image of the corresponding wound bed identified in (i) within the distal colon of an injury mouse. (iv) Corresponding NIR fluorescence image demonstrates very minimal binding of the probe toward the proliferative cells in the neutrophil cap. (v) Grayscale image captured using the polarization camera. Dotted red line marks the neutrophil cap region. (vi) Corresponding thresholded DoLP mask image identifying regions higher than established value of 10.6%. (b) Ex vivo brightfield images of wounds created via endoscopy procedure in vivo. (i,ii) Wound bed at day 4 postendoscopic injury. Dotted red line marks the boundary of the wound bed. (iii) RGB color image of the corresponding wound bed identified in (i) within the distal colon of an injury mouse. (iv) Corresponding NIR fluorescence image demonstrates minimal binding of the probe toward the wound bed (v) Grayscale image captured using the polarization camera. Dotted red line marks the boundary of the wound bed. (vi) Corresponding, thresholded DoLP mask image identifying regions higher than established value of 10.6%. (c) Ex vivo fluorescence binding pattern and histologic validation. (i,ii,iii) H&E images of disrupted epithelium in the inflamed wound region identified in (a). (iv,v,vi) Corresponding NIR fluorescence microscopy images identify proliferative cells in the wound bed. (d) Ex vivo fluorescence binding pattern and histology validation. (i,ii,iii) H&E images of wound bed in (a). (iv,v,vi) Corresponding NIR fluorescence microscopy images identify proliferative cells in the wound bed.