Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 4;5:464. doi: 10.3389/fphys.2014.00464

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 DeCant, Prinicipe, Guerra, Pasca di Magliano and Grippo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Spatial modeling of pancreatic cancer to explore cross compartmental interactions. Cre-loxP is the most widely used conditional targeting system. This is also true in models of pancreatic cancer, where it is primarily used to drive mtKRAS via a loxP-stop-loxP (LSL) cassette. However, reliance on Cre-loxP to induce a Kras mutation limits our ability to target other pertinent cell types in the tumor microenvironment. Should mtKras be induced by another system, for example a Ptf1a-FLP-driven Frt-stop-Frt (FSF) cassette, which would allow compatibility with one of the several hundred possible Cre-loxP combinations. For instance, an αSMA-Cre to explore the contributions of pancreas stellate cells to tumorigenesis, CD11b-Cre to target myeloid cells, Lck-Cre to target lymphoid cells, or Cdh5-Cre to target mature adipocytes (See Table 1).