Table 1. List of high-throughput methods for characterizing binding specificity, their advantages and disadvantages.

Method	Peptide Library Size	Quantitative	Advantages	Disadvantage
Peptide Array	10-1000’s	Semi-Quantitative	Study PTMs Generate negative binding data Easy to generate different libraries	Biased libraries (a priori knowledge) High cost of materials
Protein Microarrays	10-100’s	Quantitative	Quantitative	Protein stability Limitation in number of peptides
Phage Display	1×10ˆ10	Not quantitative	random peptides large quantities of the library low costs for production	Only natural amino acids, no PTMs high cost in DNA sequencing
Mass Spectrometry	10-100’s	Not quantitative	Physiological	Transient interactions are lost Limited to specific tissues or cells