Table 2.
Overview of studies of PrRP in vertebrate development.
| RFa and/or receptors | Species | Method | Antibody | Embryonic stages | Location of peptide/mRNA in early developing CNS | Putative functions in early development | Reference |
|---|---|---|---|---|---|---|---|
| PrRP | Guppy (Poecilia reticulata) | ir | Pol rabbit anti-salmon PrRP (56) | 0-P14 | Hyp, pituitary pars distalis at birth | Developmental role? | (57) |
| PrRP | Xenopus laevis | qPCR | – | Premetamorphosis- climax (54–65) | Transiently increased expression in brain at prometamorphosis | – | (65) |
| PrRP | Chicken pituitary | RT-PCR | – | E8–20 | Expressed in pituitary at all stages studied | – | (52) |
| PrRP | Rat | ISH, RT-PCR, ir | M 40 μl/ml P2L-1C (mature PrRP)/P2L-1T (prepro-PrRP) mouse anti human PrRP (66) | E15, E18, E20, and post-natal | NTS (E18), MRF (E20), hyp (P13) | Role in embryonic brain development? | (62) |
| PrRP + GPR10 | Rat | ISH, qPCR | – | E14-birth | PrRP: MRF, pituitary (E19), GPR10: pallidum, hippocampus, and MRF (E15–17) | Lactrotrope differentiation? | (18) |
The brain areas are generally named according to the original article. E, embryonic day; hpf, hours post-fertilization; hyp, hypothalamus; ir, immunoreactivity; ISH, in situ hybridization; M, monoclonal; MRF, medullary reticular formation; NTS, nucleus of the solitary tract in medulla; P, post-natal day; Pol, polyclonal; qPCR, quantitative PCR; RT-PCR, reverse transcription PCR.