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. 2014 Dec 4;8:148. doi: 10.3389/fnana.2014.00148

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Copyright © 2014 Klarić, Lardelli, Key, Koblar and Lewis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of npas4a mRNA in 72 hpf zebrafish embryos following PTZ treatment. Seventy-two hpf embryos were placed in embryo medium containing 3.3 mM PTZ for 20 min after which they were allowed to recover in fresh embryo medium for 30 min before being fixed for whole-mount ISH analysis using an antisense npas4a probe (B,D). Control embryos were treated as above but were placed in embryo medium that did not contain PTZ (A,C). Representative anterior (A,B) and lateral views (B,D) are shown.