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Expression of npas4a mRNA in 24 hpf zebrafish embryos following PTZ treatment. Twenty-four hpf embryos were placed in embryo medium (A) or embryo medium containing 3.3 mM PTZ (B–D) for 20 min after which they were allowed to recover in fresh embryo medium for 30 min before being fixed for whole-mount ISH analysis using an antisense npas4a probe. Representative lateral (A–C) and ventral (D) views are shown.
