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. 2014 Dec 4;8:148. doi: 10.3389/fnana.2014.00148

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Copyright © 2014 Klarić, Lardelli, Key, Koblar and Lewis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of PTZ-induced npas4a expression in the adult zebrafish brain. One year old zebrafish were placed in embryo medium containing 3.3 mM PTZ for 20 min and were then allowed to recover in fresh embryo medium for 60 min before their brains were removed for RNA extraction. Control fish were treated as above but were placed in embryo medium that did not contain PTZ. Up-regulation of npas4a mRNA expression was quantified using qRT-PCR. For each individual fish, npas4a expression was normalized to actb2 expression. Mean values and standard deviation are displayed (Control, n = 8; Moderate seizure, n = 3; Severe seizure, n = 5). Fold changes are relative to the control group which was given an arbitrary value of 1.