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Toxicity of 5-fluorocytosine to E. coli in the presence of enzymes capable of deaminating 5-fluorocytosine to 5-fluorouracil. The experiment conditions include the following: induction with 100 μM arabinose (×); 100 μM arabinose and 50 μM 5-fluorocytosine (■); 100 μM arabinose and 500 μM 5-fluorocystosine (*); 100 μM arabinose and 5 μM 5-fluorocystosine (◆). (A) Empty pBAD322c vector; (B) CodA from E. coli; (C) CodA-D314A mutant; and (D) Kpn00632.
