Table 3. Values of kcat for Enzymes Purified for this Investigation (s–1)a.
| substrate | CodA | CodA-D314A | Kpn00632 | Rsp0341 | NCgl0075 |
|---|---|---|---|---|---|
| cytosine | 33 ± 7 | 15 ± 2 | 13 ± 2 | 30 ± 5 | 0.35 ± 0.05 |
| 5-methylcytosine | NDb | 14 ± 1 | 8 ± 1 | 4.8 ± 0.8 | 1.3 ± 0.2 |
| creatinine | 0.11 ± 0.03 | 0.012 ± 0.004 | ND | ND | 32 ± 4 |
| isoguanine | 3.9 ± 0.1 | 0.68 ± 0.02 | 4.5 ± 0.5 | 0.035 ± 0.003 | 0.23 ± 0.04 |
| 5-fluorocytosine | ND | >17 | 6.5 ± 0.2 | 25 ± 3 | 0.055 ± 0.005 |
| 5-aminocytosine | ND | 24 ± 3 | 160 ± 10 | ND | ND |
| N6-methylcytosine | 0.0017 ± 0.0001 | ND | ND | ND | ND |
| 5-Hydroxymethylcytosine | ND | >3 | ND | ND | ND |
a
30 °C, pH 7.5.
b
ND: not determined.