Figure 1.
Mouse model of type I mediated food allergy to Ovalbumin. (A) Balb/c mice were sensitized three times intra-peritoneally (i.p.) with PBS (CTRL) OVA/Al(OH)3 (OVA) every 7 days and subsequently challenged with two doses of PBS or 20 mg OVA by intra-gastric (i.g) gavage. Analyses were done on day 32, three days after the last challenge. At the end of experiment, blood was removed and serum collected to measure (B) total IgE, OVA-specific IgE, (C) total IgG1 and IgG2a by ELISA in control (white circles) and OVA-allergic mice (black squares). Data are represented as mean ± SEM (n =5 mice/group). *p < 0.05, **p < 0.01.