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. 2014 Oct 14;11(12):4395–4404. doi: 10.1021/mp5004866

Figure 1.

Figure 1

Acetaminophen binds to NQO2. (a) Structures of acetaminophen and thioacetaminophen coupled to affinity matrix. (b) SDS–PAGE separation of protein eluates from thioacetaminophen (+) and control (−) columns. NQO2 detection from Western blot of the eluates is shown at bottom. (c) Thermal shift assays of APAP and AMAP with NQO2 and APAP with NQO1. See panel d for controls. (d) NQO2 thermal shift assay of acetaminophen regioisomers (2 mM), nitroacetanilide (2 mM), and NQO2 inhibitors quercetin (1 μM) and resveratrol (10 μM). AOAP is acetyl-o-aminophenol. Dicoumarol (10 μM) control for NQO1 is shown (N = 3–4 in panels c and d).