Table 1. Specific Activity of GP-pNA in the Presence vs Absence of Diprotin A in Various Cancer Cell Lines (pmol/min/μg protein, Mean ± SD; n = 3) and DPPIV Expression Determined with RT-PCR and with U95Av2 Affymetrix GeneChip.

			sp act. (pmol/min/μg)
cell line	rel RT-PCR expression	GeneChip expressiona	w/o diprotin A	with diprotin A	% inhibn
Caco-2	13.49		18.40 ± 1.17	0.29 ± 0.38	98.4
HepG2	12.71		20.16 ± 0.88	0.23 ± 0.49	98.9
IGROV1	7.92	171	11.42 ± 0.12	0.25 ± 0.12	97.8
PC-3	5.51	138	4.18 ± 0.11	0.30 ± 0.05	92.7
786-0	2.81	88	1.32 ± 0.10	0.08 ± 0.11	94.0
SK-OV-3	1.88	33	1.50 ± 0.20	ndb	100.0
SK-MEL-5	1.00	6	1.25 ± 0.02	0.19 ± 0.02	84.8

^aNovartis U95Av2 Affymetrix data on the DTP database.

^bNot detected.