Figure 4. Stabilizing effect of kifunensine on SUBEX-C57Y is dependent on the presence of N-glycans.
Protein extracts from plants transiently expressing SUBEX-C57Y-GFP lacking one (NQ1, NQ2, NQ3), two (NQ12, NQ13, NQ23) or all three (NQ123) of its N-glycans were incubated with/without kifunensine (Kif) and subjected to SDS/PAGE followed by immunoblotting. The asterisk indicates an unspecific band used as loading control.