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. 2014 Dec 5;464(Pt 3):401–411. doi: 10.1042/BJ20141057

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© 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Leaves from wild-type (wt) and os9-1 plants stably expressing SUBEX-C57Y-GFP were incubated with/without kifunensine (Kif). At the indicated time-points, proteins were extracted and analysed by immunoblotting. (B) Seedlings from wt plants stably expressing SUBEX-C57Y-GFP were incubated with/without Kif or MG132. Protein extracts were subjected to SDS/PAGE and immunoblotting. Detection of ubiquitin serves as a control for MG132 treatment. (C) Leaves from os9-1, sel1l and mns4 mns5 mutants stably expressing SUBEX-C57Y-GFP were incubated with or without Kif. (D) Leaves from wt plants stably expressing SUBEX-C57Y-GFP or the non-glycosylated SUBEX-C57Y-GFP variant NQ123 were incubated with/without Kif. Ponceau S (Ponc.) staining or anti-PDI detection served as loading controls.