Table 2.
Target gene | Denature | Anneal | Extend | Cycles |
---|---|---|---|---|
hCx30 | 94°C, 30 sec | 50°C, 60 sec | 68°C, 90 sec | 30 |
hCx36 | 94°C, 30 sec | 50°C, 60 sec | 68°C, 90 sec | 30 |
hCx43 | 94°C, 30 sec | 56°C, 60 sec | 68°C, 60 sec | 30 |
mCx30 | 94°C, 30 sec | 60°C, 60 sec | 68°C, 90 sec | 30 |
mCx36 | 94°C, 30 sec | 60°C, 60 sec | 68°C, 90 sec | 30 |
mCx43 | 94°C, 30 sec | 60°C, 60 sec | 68°C, 90 sec | 30 |
β-actin | 94°C, 30 sec | 56°C, 60 sec | 68°C, 60 sec | 30 |