Table 3.
Primary and secondary antibodies used for immunocytochemistry
| Target protein | Species | Dilution | Manufacturer | Catalogue # |
|---|---|---|---|---|
| Primary antibodies | ||||
| Connexin 30 | Rabbit | 1:250 | Zymed, Invitrogen | 71–2200 |
| Connexin 43 | Rabbit | 1:500 | Sigma–Aldrich | C6129 |
| Doublecortin | Goat | 1:250 | Santa Cruz | SC-8066 |
| Nestin | Mouse | 1:250 | Chemicon | MAB5326 |
| MAP2 | Mouse | 1:250 | Chemicon | MAB3418 |
| GFAP | Mouse | 1:500 | Sigma–Aldrich | G3893 |
| Secondary antibodies | ||||
| AlexaFluor 488-conjugated Anti-Mouse IGG | Donkey | 1:500 | Invitrogen | A21202 |
| AlexaFluor 488-conjugated Anti-Goat IGG | Donkey | 1:500 | Invitrogen | A11055 |
| AlexaFluor 594-conjugated Anti-Rabbit IGG | Donkey | 1:500 | Invitrogen | A21207 |