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. 2014 Nov 20;2(11):e12169. doi: 10.14814/phy2.12169

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — NaCl dose‐dependence and effects of aldosterone and TonEBP on decreased ENaC abundance by NaCl. (A and B) mRNA abundance of ENaC in mCCD_cl1 (A) and mpkCCD_cl4 (B) cells challenged or not (Ctl) 6 h with NaCl (350–500 mOsmol/kg). (C) mRNA abundance of ENaC in mCCD_cl1 cells challenged or not (Ctl) with aldosterone (1 μmol/L) or NaCl (500 mOsmol/kg) alone or with both stimuli for 6 h. Data is represented as fold difference over non‐stimulated cells and is expressed as the mean ± SEM of three independent experiments. (D) mCCD_cl1 cells transfected with scramble siRNA or siRNA against TonEBP (siTonEBP) were challenged or not (Ctl) with NaCl for 6 h. TonEBP, aldose reductase (AR) and ENaC mRNA abundance was analyzed by Q‐PCR. Data is represented as fold difference over non‐stimulated cells transfected with scramble siRNA and is expressed as the mean ± SEM of three independent experiments.