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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Biol Eng Trans. 2011;4(2):69–80. doi: 10.13031/2013.38508

Figure 4.

Figure 4

Endpoint fluorescence values for DNA standards subjected to non-instrumented nucleic acid amplification with assimilating probe. Standards classified as positive (black circles) and negative (white circles) for amplification describe a best-fit logistic curve (a = 11380; b = −0.6413; x0 = 1009; R2 = 0.841, RMSE = 2130) resulting in a detection limit of 861 fg DNA (vertical solid line, equivalent to 137 copies of the R. solanacearum genome). A single genome copy equivalent is represented by the vertical dashed line.