. Author manuscript; available in PMC: 2014 Dec 4.

Published in final edited form as: Biol Eng Trans. 2011;4(2):69–80. doi: 10.13031/2013.38508

Table 1.

Primer and probe sequences used for specific detection of R. solanacearum race 3 biovar 2.

	Nucleotide Sequence (5′ → 3′)
rk2208.1 Primer Set
F3	GAGAG ACATG TCCGA TTCCG

B3	GCCGA TGTCA TCAAG CTCAA

FIP	TGTGA CTTCC ACGTC AAGCG TTGCA ATCAC CGACT TCCTC A

BIP	GCGAG AAGCC CGTGT GCTTG TCACG ATTTT CGGCC AGTT

Loop B	TGCCG AAGAG CTTTT CGCCA ATCGA CT

Assimilating Probe
Label strand	FAM^[a] - ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA TGCCG AAGAG CTTTT CGCCA ATCGA CT

Quench strand	TCGGC ATCCG CATCC GCATT CGCAT CCGGG TCCTC AGCGT - BHQ^[b]

^[a]

FAM = 6-carboxyfluorescein.

^[b]

BHQ = Black Hole Quencher-1 (Biosearch Technologies, Novato, Cal.).