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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Aug 5;7(4):585–597. doi: 10.1007/s12195-014-0350-y

Figure 3.

Figure 3

Adipogenic differentiation was assessed by ORO staining of intracellular lipids. (A) Adipogenically induced samples are shown on the top row, with corresponding controls depicted on the bottom row (Scale bar = 200 μm). (B) Optical densities corresponding to eluted ORO stain indicated no significant lipid production occurred in induced samples over controls on a per sample basis. (C) When normalized to the number of cells in each sample, more robust lipid production was observed in sorted ASCs than unsorted SVF cells, ECs, pre-adipocytes, and SMCs (* p < 0.05). (D) Mean lipid droplet size measurements showed induced SVF cells and ASCs produced significantly larger lipids over control cells, which produced smaller, uniformly distributed lipids throughout the samples (* p < 0.05). Data are presented as Mean ± SD. Student’s t-test determined statistical significance.