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. 2014 Nov 24;12:110. doi: 10.1186/1477-7827-12-110

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© Bianchi et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Human CO, SFO and VO at MII stage. M-SER aggregates and MV complexes. By TEM, voluminous M-SER aggregates are seen in (a) (CO) and (b) (SFO cultured for 3–4 hours). Small MV complexes are also observable in (b). M-SER appear partially replaced by numerous and larger MV complexes after prolonged culture, as seen in (c,d) (VO cultured for 8–9 hours). Mitochondria (M) are well preserved in both SFO (b) and VO (d). Bar is: 1 μm (a,b,d); 2 μm (c).