Figure 6.
T2 relaxation times of SPIONs in the presence and absence of a protein corona at 37°C. (A) The transverse magnetization of bare SPIONs was measured for various concentrations of SPIONs (93 pM-619 pM). (B) Transverse magnetization of SPIONs coated with a soft FBS corona (67 pM-527 pM). SPION concentrations were determined with UV-Vis spectroscopy. The concentrations in (B) were slightly lower due to particle loss during preparation of coated SPIONs. (A) and (B) show representative data from measurements carried out in triplicate. (C) Transverse magnetization relaxation times of the bare (white circles) and protein-coated SPIONs (black squares) as a function of SPION concentration. Error bars show standard deviation (n=3). Lines are linear fits to the data.