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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Clin Sci (Lond). 2014 May;126(10):727–738. doi: 10.1042/CS20130385

Figure 3. Nitration of KAP-1 and ZNF274 suggests possible feedback mechanism in MAS activation by Ang-(1-7).

Figure 3

A) Coomassie stain of glutathione sepharose pull down experiments of ZNF274 and KAP-1 with nitration of the protein induced with peroxynitrite. Conditions for each pull down are shown below the gel. B) Western blot with the polyclonal anti-nitrotyrosine antibody of the samples in A. C) Proposed mechanism for maintaining MAS receptor on the cell surface with stimulation by Ang-(1-7). Activation of the receptor results in increased nitric oxide and thus nitration of the KAP-1 ZNF274 proteins preventing their inhibition of the MAS promoter activity.