Table 1. Cloning primers and PCR amplification conditions for the SOX genes.

T(A) = temperature of annealing for PCR. In some constructs a secondary digest was performed which cleaved only the control vector not containing an insert.

SOX gene	Sox group	DNA Source	Right Primer	Left Primer	T(A) °C	Restriction enzymes for cloning	Secondary digest	pEF-1 vector frame
SOX2	B1	DNASU: HsCD00329522	TCGGAATTCCCACATGTGTGAGAGGGGCA	AATGGATCCAGCATGGACAACATGATGGAGACG	60.5	BamHI and EcoRI	-	A
SOX3	B1	Thermo: MHS6278-202857278	AAAGAATTCTCCGATGTGGGTCAGCGGCA	ATAGGATCCGGAATGCGACCTGTTCGAGAGA	59.8	BamHI and EcoRI	-	A
SOX4	C	DNASU: HsCD00299790	TTGGAAGATATCGTAGGTGAAAACCAGGTTGGAGATG	TTCCAAGGATCCGCCATGGTGCAGCAAACCAAC	60.5	BamHI and EcoRV	EcoRI	B
SOX5	D	DNASU: HsCD00442638	GATGCGGCCGCGTTGGCTTGTCCTGCAATATGGTTTTCACTG	ACCGGATCCACCATGTCTTCCAAGCGACCAGCCTC	64.4	BamHI and NotI	EcoRI	C
SOX6	D	DNASU: HsCD00080539	TTGGAAGCGGCCGCGTTGGCACTGACAGCCTC	TTCCAAGAATTCGAAATGGGAAGAATGTCTTCCAAG	56.3	EcoRI and NotI	BstXI	C
SOX9	E	DNASU: HsCD00074606	TCGGAATTCCCAAGGTCGAGTGAGCTG	AATGGATCCATGGATCTCCTGGACCCC	56.7	BamHI and EcoRI	-	A
SOX14	B2	DNASU: HsCD00436531	GATGCGGCCGCCATGGCCGTAGCGTGGGC	ACCGGATCCACCATGGCCAAACCTTCAGACCAC	64.2	BamHI and NotI	-	C
SOX15	G	DNASU: HsCD00075337	TTGGAAGCGGCCGCGAGGTGGGTTAGGGGCATGGG	TTCCAAGGATCCGCCATGGCGCTACCAGGCTCC	65.4	BamHI and NotI	EcoRI	C
SOX17	F	DNASU: HsCD00303132	TCGGAATTCCACGTCAGGATAGTTGCAGTAA	AATGGATCCATGGGCAGCCCGGATG	57.5	BamHI and EcoRI	-	A
SOX30	H	DNASU: HsCD00005630	TTGGAAGCGGCCGCATCCCTGAGCACTTTTTCTTCTTCC	TTCCAAGAATTCGCCATGGAGAGAGCCAGACCC	60.2	EcoRI and NotI	EcoRV	C