Figure 3.
Meristem organization requires L-NMMA-dependent NO synthesis. A, Meristematic regions of 5-, 7-, and 10-d-old nia1 nia2 noa1 mutant and wild-type (Col-0) roots (grown in the presence or absence of 20 μm L-NMMA). Arrowheads indicate the end of the meristem and the beginning of the elongation/differentiation zone. B, CycB1;1pro:GUSDB expression in 5-, 7-, and 10-d-old noa1 mutant and wild-type (Col-0) seedlings grown in the presence or absence of 20 μm L-NMMA. Bars = 50 µm. C, QC/columella stem cells (CSC) disorganization and starch accumulation in 5-d-old nia1 nia2 noa1 mutant and wild-type (Col-0) seedlings grown in the presence or absence of 20 µm L-NMMA using modified pseudo-Schiff PI stain. Red, blue, and green arrowheads indicate QC, CSC, and columella cells, respectively.