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. 2014 Nov 30;22(6):540–546. doi: 10.4062/biomolther.2014.081

Fig. 5.

Fig. 5.

Ketotifen reduced MMP-9 expression of MDA-MB-231 and HT-1080 cells. (A) Effect of ketotifen on expression of MMP-9 in MDA-MB-231 cells. MDA-MB-231 cells were treated with ketotifen (1, 5, 25 μM) for 24 h. (B) Effect of ketotifen on expression of MMP-9 in TPA-treated HT-1080 cells. HT-1080 cells were treated with ketotifen (1, 5, 10, 25 μM) and with TPA (20 nM) for 24 h. Whole-cell lysates (10 μg) were prepared, the protein level was subjected to 10% SDS-PAGE, and the expressions of several proteins were determined by western blotting. (C) Effect of ketotifen on MMP-9 activity in HT-1080 cells. To test the activity of MMP-9, HT-1080 cells were treated with ketotifen (1, 5, 10, 25 μM) and with TPA (20 nM) for 24 h. Conditioned media were collected, and gelatin zymography was performed as described in Materials and Methods.