Fig. 3.

Effect of R. fasciculatum on the DNCB-induced dermatitis and serum IgE level. (A) The BALB/c mice (n=6) were sensitized with 100 μl of 0.1% DNCB in acetone-olive oil (3:1) or vehicle (acetone/olive oil=3:1) applied to the dorsal skin twice each week for a total period of 5 weeks. After 3 weeks, R. fasciculatum (200 mg/kg) was orally administered 2 week prior to the end of the experiment. (B) Blood samples were collected and then levels of serum IgE in the indicated groups were measured using ELISA method. Statistical evaluation of the results was performed by independent t-test. Each datum represents the means ± S.D. of three independent experiments (^#p<0.05 vs. control group, ^*p<0.05 vs. DNCB -treated group).