Fig. 5.

Effect of R. fasciculatum on the NF-κB activation in the nuclei of LPS-stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.5 mg/ml) for 1 h and then stimulated with LPS for 2 h. (A) Nuclear extracts were prepared as described in the Materials and Methods section and evaluated for RelA/p65 via Western blot analysis. (B) The relative levels of NF-κB were represented. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (^#p<0.05 vs. control, ^*p<0.05 vs. LPS alone).