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The mRNA expression of marker genes for bone formation. The mRNAs of Collagen type I and OCN from heat-, and ibanronate-treated rats and control, respectively were extracted and measured by RT-PCR (A). The histogram depicts the mRNA of Collagen type I and osteocalcin (OCN) compared to β-actin (B). Asterisk (*) indicates a significant difference (p<0.05) compared with the control (#). Each column represents the mean ± S.E.M. of three or four separate experiments.
