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. 2014 Nov 30;22(6):563–569. doi: 10.4062/biomolther.2014.111

Table 1.

Primer sequences and conditions for RT-PCR

Target genes (Accession number) Primers Forward/Reverse PCR condition

Tm(°C) Cycles Size(bp)
Collagen 1 5′-ttctcctggtaaagatggtgc-3′ 50 35 254
(NM_007742) 5′-tgttaaaggtgatgctggtcc-3′
OCN 5′-cagcttggtgcacacctagc-3′ 58 30 242
(NM_001032298) 5′-ggagcagtgtcacgttaacct-3′
β-actin 5′- ttctacaatgagctgcgtgt-3′ 60 25 456
(NM_007393) 5′- ctcatagctcttctccaggg-3′