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. 2014 Nov 29;14:99. doi: 10.1186/s12896-014-0099-3

Table 1.

Formation of mAb dimer, oligomer, fragment and large aggregates using the different induction methods

mAb1 mAb2
Dimer (%) Oligomer (%) Zave (nm) Dimer (%) Oligomer (%) Fragment (%) Zave (nm)
pH
3 1.8 - 34.2 ± 1.8 1.7 1.8 - 39.6 ± 2.4
4 1.9 - 32.5 ± 3.3 2.1 0.9 - 49.5 ± 3.5
5 2.3 - 84.3 ± 15.0 2.0 0.9 3.5 144.9 ± 35.8
6 2.6 - 85.7 ± 1.9 3.5 0.6 5.7 47.9 ± 1.3
7 3.1 - 51.1 ± 5.3 4.0 0.5 4.5 45.7 ± 0.8
8 3.4 - n. a. 6.3 0.6 6.3 n. a.
NaCl
Control 0.6 - 33.7 ± 5.3 1.1 - - 33.2 ± 0.2
500 mM 1.3 - 23.8 ± 0.2 3.0 - - 41.8 ± 1.9
1 M 1.7 - 24.8 ± 0.6 4.2 - - 56.6 ± 8.8
1.5 M 2.0 - 24.2 ± 1.6 4.6 - - 78 ± 9.2
FT
Control 0.6 - 19.7 ± 1.1 1.4 - - 10.6 ± 1.1
16.0 11.3 20.7 ± 1.8 17.1 3.6 - 14.4 ± 0.1
20.2 16.3 26.6 ± 1.9 19.3 3.9 - 12.9 ± 0.5
22.7 11.0 27.3 ± 2.8 21.0 6.6 - 12.1 ± 0.5