Figure 5. Posttranslational change in regulation after gene duplication in Swi5 and Ace2.

A) Schematic of the gene tree relating the Ace2/Swi5 paralog pair with diagram of protein features found in proteins from different yeast species. Bolded species name indicate cloned genes assayed for localization in S. cerevisiae. The nuclear localization signal characterized in Swi5 is putatively altered and may not be functionally homologous in Candida and Ace2, but this difference was not predicted in our analysis (see Discussion and S3 Figure). B) Green-fluorescent protein tagged genes cloned from the labeled species were assayed for their localization in unsynchronized S. cerevisiae cells. Two representatives of each pre-/post-WGD genes were assayed. Orange and blue arrows indicate representative bud and mother nucleus pairs. C) The fluorescence intensity of the nucleus in cells expressing the labeled proteins was quantified, and mean difference of the intensity (bud-mother) is used as the measure of asymmetry (unfilled bars). Error bars show 95% confidence interval of the mean. Stars indicate 5% statistical significance. Red double arrow illustrates the duplication event. Scer: S. cerevisiae, Cgla: C. glabrata, Zrou: Z. rouxii, Lwal: L. waltii, Lklu: L. kluyveri, Calb: C. albicans.