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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Curr Biol. 2014 Nov 6;24(23):2749–2757. doi: 10.1016/j.cub.2014.09.048

FIGURE 1.

FIGURE 1

Thymus extract is more efficient at depolymerizing single actin filaments than a normalized amount of recombinant cofilin. (A) Quantitative western blot showing standard amounts of recombinant cofilin (0.01–0.5 µg) (left lanes, increasing order) & fixed amounts of thymus extract. (B) Cofilin severs only across a narrow range of concentrations (left graph), with activity peaking at approximately 1 µM. Thymus extract is roughly 10-fold more effective at severing than the peak cofilin severing concentration (right graph) Error bars represent S.D. n= at least 2 movies. (C) Frames from time lapse movies showing that when normalized to a cofilin concentration of 5 µM, filaments are stable in the presence of pure cofilin (top panel), whereas they are disassembled within 60s with thymus extract (bottom panel). Scale bar= 2 µM.