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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Curr Biol. 2014 Nov 6;24(23):2749–2757. doi: 10.1016/j.cub.2014.09.048

FIGURE 2.

FIGURE 2

Aip1 can sever filaments saturated by cofilin. (A) Frames from time lapse movies showing the dynamics of filaments in the presence of a saturating amount of cofilin+ CapZ (upper panels) or cofilin + Aip1 (lower panels). Filaments fragment in the presence of Aip1 only, not cofilin alone or cofilin + CapZ. (B) Quantitation of severing rates of filaments in the presence of saturating amounts of cofilin. No severing events were detected in the presence of 25 µM cofilin alone or cofilin + 100 nM CapZ. Severing rates increased with increasing concentrations of Aip1 (n=3 movies, error bars represent S.D.). Scale bar= 1 µM.