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Two-photon ISIM enables visualization of subnuclear chromatin structure throughout nematode embryos. (a) Selected slices at indicated axial distance from the coverslip, through a live nematode embryo (bean stage). Scale bar: 10 μm. (b) Higher magnification views of yellow rectangular regions in (a), emphasizing subnuclear chromatin structure throughout the imaging volume. Scale bar: 2 μm. All images have been deconvolved. See also Media 1.
