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Curcumin-loaded silk hydrogel films were immersed in either PBS buffer, pH 7.4 (A, B), or PBS buffer supplemented with Tween 80 and methanol (C,D) for release study. The concentration of released curcumin in solution was determined by absorbance at 424 nm. A,C, curcumin concentrations (μM) determined in the released medium. B,D, cumulative release calculated based on original curcumin loaded to the films. N=4.
