Figure 3. dMRP4 is required for JNK-mediated gene expression under oxidative stress.

qt-PCR analysis of mRNA isolated from WT flies (w¹¹¹⁸) after exposed to 10 mM paraquat (PQ) for indicated time points. (A–D) Relative mRNA levels were compared between w¹¹¹⁸ and dMRP4 to the respective controls (no paraquat feeding) where the basal values were set at 1.0. (E) Relative mRNA levels of dMRP4 from flies carrying actGS-Bsk^DN were compared between RU486 feeding (+RU, 150 ug/ml) and non-feeding groups [75]. (F) Relative mRNA levels of dMRP4 from flies carrying actGS-Hep^Act were compared between RU486 feeding (+RU, 150 ug/ml) and non-feeding groups [75]. puc expression served as a marker for JNK activity in response to paraquat. Data was presented as means ± SD from 3–5 independent experiments. Student's t-test: * p<0.05, ** p<0.01, *** p<0.001. ns: No significance (p>0.05).