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. 2014 Dec 4;10(12):e1004844. doi: 10.1371/journal.pgen.1004844

Figure 5. dMRP4 affects lifespan.

Figure 5

(A) Lifespan of adult flies lacking dMRP4. Survival was presented as mean of at least 300 males with different genotypes. Homozygous dMRP4 mutant flies lived significantly shorter than their sibling controls (Log-rank test, p<0.0001): the mean lifespan (50% mortality) was 45 days for dMRP4M2/M2 and 45 days for dMRP4M2/M1, respectively, compared to 66 days for wild-type control (w1118); the maximum lifespan (90% mortality) was 63 for dMRP4M2/M2 and 60 days for dMRP4M2/M1, respectively, compared to 78 days for wild-type control. (B) Analysis of age-specific mortality. The log cumulative hazard plots were presented for different genotypes of survival data. The ratio of slopes from Partial Slopes Rank-Sum Test: w1118 vs dMRP4M1/M1 = 1.24 (p = 0.2005), w1118 vs dMRP4M2/M2 = 1.32 (p = 0.0947), dMRP4M1/M1 vs dMRP4M2/M2 = 1.07 (p = 0.7182). (C) Lifespan of adult flies with genotype tub5GS>dMRP4 (tubulin5-GS-Gal4/EP3177) between treatments: −RU486 group, mean = 64 days, maximum = 78 days (n = 383), +RU486 group (100 ug/ml), mean = 74 day, maximum = 84 (n = 427), and +RU486 group (20 ug/ml), mean = 70 days, maximum = 82 days (n = 438). The cohort was derived from a combination of two independent cohorts (about 200 flies for each cohort) which were conducted over different time periods, and the individual cohorts were similar to each other. (D) Effects of RU486 treatment on control groups. tub5GS-Gal4/+ (−RU486), mean = 62 days (n = 300) and tub5GS-Gal4/+ (+RU486 100 ug/ml), mean = 62 days (n = 300). (E) Lifespan of adult flies with genotype S106>dMRP4 (S106-Gal4/+; EP3177/+) between treatments: −RU486, mean = 60 days (n = 408), or +RU486, mean = 62 days (n = 460). (F) Effects of RU486 treatment on control groups. S106-Gal4/+ (−RU486), mean = 54 days (n = 300) and S106-Gal4/+ (+RU486 100 ug/ml), mean = 55 days (n = 300). (G) Lifespan of adult flies with genotype da>dMRP4 was compared to the parent controls. The mean lifespan of these flies was 62 days for da-Gal4/+ (n = 340), 61 days for dMRP4/+ (n = 320), and 45 days for da>dMRP4 (n = 380). The maximum lifespan was 77 days for da-Gal4/+, 77 days for dMRP4/+, and 56 days for da>dMRP4. (H) qt-PCR analysis of the dMRP4 expression driven by da-Gal4.