Table 4. Candidate reference genes for normalization listed according to their expression stability calculated by the NormFinder program.
Ranking order | Gene name | Stability value |
1 | UBC | 0.365 |
2 | PPIA | 0.370 |
3 | HPRT1 | 0.436 |
4 | H3F3A | 0.473 |
5 | GAPDH | 0.480 |
6 | TBP | 0.566 |
7 | GUSB | 0.586 |
8 | POLR2A | 0.653 |
9 | ACTB | 0.657 |
10 | B2M | 0.682 |
High expression stability is indicated by a low stability value as an estimation of both the intra- and the intergroup variation of individual gene, according to the model-based approach developed by Andersen et al. [17].