Table 3. Polymorphic sites allowing differences between ITS-2 and ITS1 rDNA haplotypes of T. infestans samples analysed from Bolivia, Peru, Chile and Argentina and those available in GenBank.
ITS-2 | ||
GenBank Acc. No. | Haplotype code | Variable positions |
222 2334444444 4444444444 4444444444 444444444 | ||
5557777222 2454444555 5555555666 6666666777 777777788 | ||
4675678678 9726789012 3456789012 3456789012 345678901 | ||
AJ576051 | T.inf-GT1 | T------AAA TCAATATTTA AAAAGAAAAG AGATGCGCAA TCATTTTTT |
AJ576051 | T.inf-H1 * | ................................................. |
AJ576054 | T.inf-GT2 | A—ATAT.....T.................................... |
AJ576054 | T.inf-H2 * | A—ATAT.....T.................................... |
AJ576052 | T.inf-GT3 | AATATAT.....T.................................... |
AJ576053 | T.inf-GT4 | A—AT----- -....................................... |
AJ576055 | T.inf-GT5 | A—ATAT........................................... |
HQ333211 | T.inf-ITS2Hap1 | .------....................--- ---------- --------- |
HQ333212 | T.inf-ITS2Hap2 | A—AT—....................--- ---------- --------- |
HQ333213 | T.inf-ITS2Hap3 | A—AT—....T...............--- ---------- --------- |
HQ333214 | T.inf-ITS2Hap4 | A—ATAT.....T..............--- ---------- --------- |
AY860387 | ITInf72 | AATATAT.....T.................................... |
AY860388 | ITInf74 | A------......------- ---------- ---------- --------- |
*haplotypes from present paper; variable positions = numbers (to be read in vertical) refer to variable positions obtained in the ITS-2 and ITS-1 alignments obtained with MEGA 6.0; symbols: identical positions = .; indel = -; indel position in 3′ end of the alignment = not sequenced. Haplotypes that seem to be identical: T.inf-GT1 = T.inf-ITS2Hap1; T.inf-GT2 = T.inf-ITS2Hap4; T.inf-GT3 = ITInf72.