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. 2014 Dec 4;10(12):e1004839. doi: 10.1371/journal.pgen.1004839

Figure 3. Loss of Heterozygosity (LOH) assay.

Figure 3

A. A schematic representation of the LOH assay in heterozygous diploid yeasts (YPH45×YJM789). YPH45-CHR3 and YJM789-CHR3 are represented by black and red lines, respectively. Location of the pTET-lys2-GTOP or –GBTM cassette on the left arm of CHR3 is indicated by the hashed box. Green boxes indicate the approximate locations of the heterozygous SNP markers listed in Table S1. Telomeres are represented as “tgtgtg”. Hemizygous URA3 and G418R markers present only on the YPH45-CHR3 homolog are indicated by blue boxes. The top panel and the bottom two panels represent the parental 5-FOAS diploid and LOH Class E events as described in the text and in Table 2, respectively. LOH Class E events initiating at the pTET-lys2-GTOP or –GBTM cassette either by recombination or deletion will result in the same RFLP-SNP pattern but in the difference in size alterations of the YPH45 CHR3. B. The orientation of pTET-lys2-GTOP or –GBTM cassette insertion relative to ARS306 in SAME or OPPO configuration.