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. 2014 Dec 4;10(12):e1004839. doi: 10.1371/journal.pgen.1004839

Figure 6. The effect of replication direction on the gene conversion rate.

Figure 6

A. A schematic representation of the gene conversion assay. The pTET-lys2-GTOP cassette (indicated by the hashed box) is located in “Head-on” orientation relative to ARS306. In the ars306Δ strain, the closest origin of replication is ARS305 from which replication proceeds in a co-directional orientation with the transcription from pTET. The distances (in kb) are approximate and not to scale. B. The rates of gene conversion at pTET-lys2-GTOP or –GBTM cassette. Transcription and replication are in the “Head-on” orientation when ARS306 (gray bar) is present and in the “Co-directional” orientation in ars306Δ strains (hashed bar). The 95% confidence intervals are indicated by the error bars.