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| PCR Fragments | G4 Construct | |||||
| A (URA3) | B (CAN1) | C (lys2 * ) | D (G418R) | E (PCM1) | pTET-lys2-GTOP (N = 30) | pTET-lys2-GBTM (N = 23) |
| + | + | + | + | + | 0 | 5 |
| − | − | + | + | + | 3 | 5 |
| − | − | − | + | + | 27 | 9 |
| − | − | − | − | + | 0 | 4 |
*PCR primers were designed to anneal to LYS2 sequence upstream and downstream of the embedded Sμ fragment (GTOP or GBTM). See Fig. 1A for the approximate location of the PCR fragments analyzed. + indicates the presence of PCR product and − indicates the absence.
