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. 2014 Dec 4;10(12):e1004841. doi: 10.1371/journal.pgen.1004841

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) and (B) A schematic diagram of the MIR162b (A) and pre-miR162b (B) used in vitro processing assay. (C) and (D) The amount of miR162b produced from MIR162b and pre-miR162b were reduced in prl1-2. Proteins were isolated from inflorescences of prl1-2 and Col and incubated with MIR162b or pre-miR162b. The reactions were stopped at various time points as indicated in the picture. (E) and (F) Quantification of miR162b production in prl1-2 compared to that in Col. Quantification analysis was performed at 80 min. The radioactive signal of miR162 were normalized to input and compared with that of Col. The amount of miR162 produced in Col was set as 1. The value represents mean of three repeats (*** P<0.001; t-test).