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. 2014 Dec 4;10(12):e1004841. doi: 10.1371/journal.pgen.1004841

Figure 7. PRL1 binds pri-miRNAs in vitro and in vivo.

Figure 7

(A) Proteins used for in vitro RNA binding assay. Purified MBP and MBP-PRL1 proteins were resolved on SDS-page gel and stained by Coomassie Blue. The lower bands in MBP-PRL1 line are degraded MBP-PRL1 since they can be detected by anti-MBP antibody. M: marker. (B) PRL1 binds MIR162b, pre-miR162b and ssRNA in vitro. MIR162b, pre-miR162b and ssRNA were produced by in vitro transcription. SsRNA: single stranded-RNA; DsRNA: double stranded-RNA. (C) PRL1 binds pri-miRNAs in vivo. NoAb: No antibody control. Transgenic plants harboring pPRL1::PRL1-YFP, pTGH: TGH-YFP or YFP transgene were used for RIP assay with anti-YFP anti-bodies. Ten percent immunoprecipitates and two percent input proteins were analyzed by western blot. Five percent RNAs were used as input RNA.